Living Mysteries: This complex beast lurks on lobster whiskers | Science News for Students

Living Mysteries: This complex beast lurks on lobster whiskers

The pandora’s tiny body is a lean and mean marvel of evolution
Dec 13, 2018 — 6:45 am EST
pandora lobster

Symbion pandora (left) is a tiny animal that lives on the mouth whiskers of lobsters. Smaller than a grain of salt, it devours  leftover crumbs from its host’s meal. Pandora lives on only the Norway lobster (right). At least two other Symbion species reside on other lobsters.

From left: Reinhardt Møbjerg Kristensen; Hans Hillewaert/Wikimedia Commons (CC BY-SA 4.0)

One of the world’s weirdest beasts was discovered hiding on the whiskers of a lobster. It’s called Symbion pandora. And a single lobster can host thousands of pandoras. If you’ve ever eaten lobster, you may even have dined on these critters without knowing it.

The whiskers around a lobster’s mouth, on its underside, are speckled yellow-white. Though teeny, those speckles are actually a vast city of pandoras.

Under a microscope, the individual critters take shape. They hang onto a lobster’s whisker like plump little pears on a tree branch. Each is smaller than a grain of salt. But up close, a pandora appears terrifying — like an angry vacuum cleaner. It has a sucker mouth surrounded by little hairs.

When a lobster eats a worm or fish, these little monsters devour the crumbs. A single blood cell barely squeezes down a pandora’s throat.

A closer look at an individual pandora reveals that it is actually a whole little family. Inside, next to its stomach, is a baby. And sitting on pandora’s back is a pouch that holds two hitchhiking males.

This species is one of the smallest animals known — and the little male is smallest of all pandoras. Its body contains just a few dozen cells. And yet it makes the most of those cells. It has a brain and other important organs.

When it comes to how small an animal can be, “this is really close to the limit,” says Reinhardt Møbjerg Kristensen. He’s a zoologist at the University of Copenhagen in Denmark. “We are down to the smallest, smallest invertebrate [animal] that we have on Earth.” (By invertebrate, he’s referring to animals lacking a backbone. These account for about 95 percent of all animals.)

Pandora shows scientists how evolution can strip a creature’s body down to almost nothing. Yet this tiny body is anything but simple. It is actually quite advanced.

Remote island

Scientists first noticed these little animals on lobsters’ whiskers in the 1960s. No one knew what they were. So Claus Nielsen preserved the animals for future study. He was a zoologist at the Marine Biological Laboratory in Helsingør, Denmark. He took some lobster whiskers, with the creatures attached, and embedded them in clear plastic.

norway lobster
Norway lobsters are a popular seafood. Scientists have found that their mouth whiskers have microscope tagalongs. 
Lucas the Scot/Wikimedia Commons

It was not until 1991 that Nielsen handed that plastic to Peter Funch. Funch was a graduate student at the time, working with Kristensen.

Funch would study this animal, nonstop, for the next five years. He took detailed pictures of it, each magnified several thousand times. He traveled to remote islands in the Atlantic Ocean for a month at a time. There, he bought freshly caught lobsters from local fishermen. He trimmed off the creatures’ whiskers and collected live pandoras. Then he watched through a microscope as the little critters ate and grew.

Funch remembers these trips as enjoyable, but a lot of work. He often worked until 3 o’clock in the morning. They were “very, very long days,” he says. “You’re trying to solve this mystery and you’re just totally into it.”

He and Kristensen named this newly discovered animal species Symbion pandora. They named it after Pandora’s box. This little box, in Greek mythology, was a gift from the god Zeus. The box was stuffed full of death, disease and many other complicated problems — just as the little pandora, on a lobster’s whisker, also turned out to be surprisingly complicated, despite its small size.

Baby of the month

Scientists find new species all the time. They usually belong to groups of species that are already known — like a new kind of frog, or a new type of beetle. But this new species, S. pandora, was far more mysterious. It was not closely related to any known animal.

Funch and Kristensen also realized that it has a surprisingly complicated life. For one thing, not all of these animals are alike. Only a few grow up to be “big pandoras” that eat and make babies.

Pandora also reproduces in a weird way. Big pandoras, which are neither male nor female, usually have a baby growing inside them. Each makes one baby at a time. But it can make three different types of babies. And which type it makes depends on the time of year.

pandora crowd
A whole city of pandora, with thousands of the tiny animals, can live on the mouth whiskers of a single lobster. This scanning electron microscope image has magnified the animals about 150 times.
Peter Funch and Reinhardt Møbjerg Kristensen

During fall, a big pandora will make copies of itself. The newborns then sit down on another lobster whisker. They open their sucker mouths and start feeding. Pretty soon they start making their own babies.

In early winter, all of these big pandoras start making male babies. As each male is born, it crawls away and finds another big pandora. It glues itself to that big pandora’s back. And then, something strange happens. This glued-down male begins growing two smaller males inside it. Pretty soon, the first male is nothing but a hollow pouch glued to a big pandora’s back. And hiding inside the pouch are two “dwarf males.” These are tiny — just one one-hundredth the size of big pandora. The dwarf males stay inside the pouch, waiting for females to be born.

By late winter, all of the big pandoras have dwarf males waiting on their backs. Now, they switch to making female babies. Funch could tell these babies were females because each had what looked like a big beach ball inside. That “beach ball” was an egg cell — ready to be fertilized by a male.

Funch took several years to figure out the complex story of how pandoras reproduce. By 1998, he had finished his doctoral degree and become a professor of zoology at Aarhus University in Denmark. It would be up to someone else to discover pandora‘s next surprise. That someone was Ricardo Cardoso Neves. He started as Kristensen’s new graduate student in 2006.

Shrinking boy

Neves set out to count how many cells make up the dwarf male’s body. He marked them with a dye that binds to a cell’s nucleus (NOO-klee-us). The nucleus is the bag that holds a cell’s DNA. Each cell has one nucleus, so counting the nuclei (NOO-klee-eye) told him how many cells there were. And the result shocked him.

A tiny mosquito has more than a million cells in its body. One of the world’s smallest worms, called C. elegans, has a body shorter than the thickness of a penny. It has about 1,000 cells. But a dwarf male pandora has just 47.

pandora mouth
This closeup of a pandora’s mouth shows it is surrounded by tiny hairs called cilia. The animal eats by twirling these cilia, which pulls little bits of food into its mouth. A single blood cell from a fish or a crab can barely squeeze down a pandora’s throat.
Peter Funch and Reinhardt Møbjerg Kristensen

Most of those cells — 34 of them — form its brain, Neves found. Another eight cells make up its glands. Those are small organs that ooze out gooey mucus to help the male crawl. Two more cells form the male’s testes. Testes make the sperm that fertilize a female’s egg. The remaining three cells may help the animal feel its surroundings.

So the adult male is incredibly compact. But as Neves studied it, he discovered an even bigger surprise. The male starts its life with a lot more cells — some 200! As it grows up inside its little pouch, it does the opposite of what most animals do, whether humans or dogs. The body of the dwarf male shrinks in size.

Most of its cells lose their nuclei and their DNA. That DNA is precious cargo. It holds the directions for building a cell. Without it, a cell can no longer grow or repair damage. A cell may live for a while without its DNA — but not for long.

So getting rid of the nuclei is an extreme step. But Neves realized that male pandoras had a good reason for doing this. “They get rid of the nuclei just because they don’t have enough space,” he says.

The males spend most of their life hiding inside the little pouch that sits on the back of a big pandora, he points out. It’s a tight fit. But by losing so much DNA, the male shrinks its body size by almost half. That lets two males fit inside the pouch.

And that’s important because any male not in a pouch will get swept away.

A lobster’s mouth whisker is “a dangerous place to be,” Neves explains. As the lobster eats, its whiskers swish rapidly back and forth through the water. To survive on a whisker, a creature must hold on tight. Any that don’t will get thrown off, like a monkey blown out of a tree by a hurricane.

Big pandoras glue themselves permanently to their whisker. The little dwarf males and females use the big pandoras for shelter. The female stays safely inside big pandora’s body. Males stay tucked in the pouch glued to a big pandora’s back.

Funch thinks that the males emerge only once, when it is time to mate. One day in 1993, he was watching a big pandora with a baby female inside its body. Suddenly, the female was moving. She wiggled out of her usual chamber and into big pandora’s gut. The gut is the tube that carries digested food from the stomach to the anus, where the poop comes out.

Young mother

As Funch watched, the big pandora’s muscles squeezed around its gut and pushed the female through — the same way it squeezes out poop. Slowly, the female emerged out of the anus.

The female’s back end came out first. Sitting inside her back end was the big, round egg cell. It was ready to be fertilized by a male. And of course the two males were waiting right there in their pouch.

Funch never quite saw the animals mate. But he has an idea about what happened next. He thinks the two males burst out of their shelter. One mates with the female as she was being born. So by the time she was all the way out, her egg is already fertilized. She then can glue herself to another whisker and let the baby inside her grow.

In this situation, Funch and Neves say, it makes sense that the male is so small. He has no stomach or mouth because those would take up too much room in the pouch. He doesn’t need to live longer than a few weeks. And most of that short life is spent waiting, saving energy. His life has one purpose: Reach the female. Once he mates, he can die. Having two males in the pouch ups the chance that one will succeed.

pandora pair
This electron microscope picture of two pandoras on a lobster whisker shows the hair-like cilia that surround their mouths. The pandora on the left also has a sack on its side, which holds two tiny dwarf males.
Peter Funch and Reinhardt Møbjerg Kristensen

There are other cases where evolution has produced dwarf males. One tiny stinging wasp called Megaphragma (Meh-guh-FRAG-muh) is only two tenths of a millimeter long (less than one one-hundredth of an inch). It is actually smaller than a single-celled amoeba (Uh-MEE-buh). The male starts out with about 7,400 nerve cells. But as it matures, it loses the nuclei and DNA from all but 375 of those cells. This male lives only five days.

But the pandora dwarf male, with only 47 cells, slims down to an even greater extreme. It “is something unique in the animal kingdom,” says Neves. “It’s a fantastic organism.”

Pocket watch

Even a big pandora is smaller and has fewer cells than just about any other animal. But it would be a mistake to call it primitive. Consider a pocket watch. It’s smaller than a grandfather clock. But is it simpler? The pocket watch’s small size actually makes it more complicated. Every gear and spring has to fit perfectly inside its small case. The same is true for the pandora. This animal, says Kristensen, “must be very advanced.”

Evolution can sometimes turn small, simple bodies into large and complicated ones. That’s what happened with apes and humans over the last 20 million years. Our bodies, brains and muscles got bigger.

But just as often, evolution pushes animals the other way. It pushes them toward having weaker bodies, smaller brains and shorter lives.

green pandora
Pandoras may be tiny, but that doesn’t mean they are simple.
Reinhardt Møbjerg Kristensen

Evolution is all about surviving long enough to produce offspring. And sometimes the best way to do that is to keep bodies small and compact. With pandora, the species’ evolution was shaped by its need to survive a terrible disaster that happens every so often.

Once or twice per year, the entire pandora city dies. This happens because the lobster sheds its shell — including its mouth whiskers. On that day, the entire city of pandoras that was glued to the whiskers now drops to the dark seafloor. Without any of their host’s leftovers to eat, these pandoras starve.

Life boats

Pandora’s odd life style evolved so that it could produce as many babies as possible to survive this disaster. Big pandoras stay glued to a lobster’s mouth whiskers. They eat and use the energy in a lobster’s food scraps to make little males and females, each in their own season. And big pandoras keep their offspring together so that they can mate — and produce a different type of baby. One that will survive.

After the female emerges with her fertilized egg, she glues herself to another whisker. The baby grows inside her. Before that baby is even born, says Funch, it “eats its own mother.”

By the baby’s birth, its mother is nothing but a hollow husk. From its mother, the baby gains enough energy to grow strong muscles. Unlike the big pandora, and unlike the male and female that mated to produce it, this baby is actually a strong swimmer.

Such strong little swimmers leave the dying pandora city. They are like thousands of life boats fleeing a sinking ship. They swim until a few lucky ones find a new lobster. There, they glue themselves onto a mouth whisker. They now change shape, morphing into new big pandoras. They grow mouths and stomachs. They begin to eat and to make babies. So begins a new pandora city.

This “is just an amazing group of organisms,” says Gonzalo Giribet. He’s a biologist at Harvard University in Cambridge, Mass. He studies unusual spiders, sea slugs and other creepy crawlies. He has watched with great interest as the pandora story has unfolded over the last few years.

Pandoras show scientists how evolution can solve common problems in surprising ways, he says. “It’s almost like a great piece of art.”

Pandoras have many lessons to teach scientists. But the greatest might be not to overlook what’s in plain sight. This animal was living in a place that people thought they knew well: on lobsters that people eat every day. “Imagine how ridiculous it is,” says Giribet. “It teaches us about biodiversity, and how little we know.”

Power Words

(more about Power Words)

amoeba     A single-celled microbe that catches food and moves about by extending fingerlike projections of a colorless material called protoplasm. Amoebas are either free-living in damp environments or they are parasites.

anus     The opening at the end of an animal's digestive system through which solid waste leaves the body.

ape     A group of rather large “Old World” primates that lack a tail. They include the gorilla, chimpanzees, bonobos, orangutans and gibbons.

Atlantic     One of the world’s five oceans, it is second in size only to the Pacific. It separates Europe and Africa to the east from North and South America to the west.

beetle     An order of insects known as Coleoptera, containing at least 350,000 different species. Adults tend to have hard and/or horn-like “forewings” which covers the wings used for flight.

biodiversity     (short for biological diversity) The number and variety of species found within a localized geographic region.

biology     The study of living things. The scientists who study them are known as biologists.

cell     The smallest structural and functional unit of an organism. Typically too small to see with the unaided eye, it consists of a watery fluid surrounded by a membrane or wall. Depending on their size, animals are made of anywhere from thousands to trillions of cells. Most organisms, such as yeasts, molds, bacteria and some algae, are composed of only one cell.

cilia     (singular cilium) Small hairlike features that occur on the surface of some cells and larger tissue structures. They can move and their wavelike motion can propel liquids to move in a particular direction. Cilia play an important role in many biological functions throughout the body.

DNA     (short for deoxyribonucleic acid) A long, double-stranded and spiral-shaped molecule inside most living cells that carries genetic instructions. It is built on a backbone of phosphorus, oxygen, and carbon atoms. In all living things, from plants and animals to microbes, these instructions tell cells which molecules to make.

doctoral degree     Also known as a PhD or doctorate, it is an advanced degree offered by universities — typically after five or six years of study — for work that creates new knowledge. People qualify to begin this type of graduate study only after having first completed a college degree (a program that typically takes four years of study).

egg     The unfertilized reproductive cell made by females.

evolution     (v. to evolve) A process by which species undergo changes over time, usually through genetic variation and natural selection. These changes usually result in a new type of organism better suited for its environment than the earlier type. The newer type is not necessarily more “advanced,” just better adapted to the particular conditions in which it developed. Or the term can refer to changes that occur as some natural progression within the non-living world (such as computer chips evolving to smaller devices which operate at an ever faster speed).

family     A taxonomic group consisting of at least one genus of organisms.

fertilize     (in biology) The merging of a male and a female reproductive cell (egg and sperm) to set in create a new, independent organism.

gland     A cell, a group of cells or an organ that produces and discharges a substance (or “secretion”) for use elsewhere in the body or in a body cavity, or for elimination from the body.

graduate student     Someone working toward an advanced degree by taking classes and performing research. This work is done after the student has already graduated from college (usually with a four-year degree).

gut     An informal term for the gastrointestinal tract, especially the intestines.

host      (in biology and medicine) The organism (or environment) in which some other thing resides. Humans may be a temporary host for food-poisoning germs or other infective agents.

invertebrate     An animal lacking a backbone. About 90 percent of animal species are invertebrates.

marine     Having to do with the ocean world or environment.

mature     (adj.) Connoting an adult individual or full-grown and fully developed (non-juvenile) form of something. (verb) To develop toward — or into — a more complex and  full-grown form of some individual, be it a plant, animal or microbe.

microscope     An instrument used to view objects, like bacteria, or the single cells of plants or animals, that are too small to be visible to the unaided eye.

mucus     A slimy substance produced in the lungs, nose, digestive system and other parts of the body to protect against infection. Mucus is made mainly of water but also includes salt and proteins such as mucins. Some animals use mucus for other purposes, such as to move across the ground or to defend themselves against predators.

muscle     A type of tissue used to produce movement by contracting its cells, known as muscle fibers. Muscle is rich in protein, which is why predatory species seek prey containing lots of this tissue.

nerve     A long, delicate fiber that transmits signals across the body of an animal. An animal’s backbone contains many nerves, some of which control the movement of its legs or fins, and some of which convey sensations such as hot, cold or pain.

nucleus     Plural is nuclei. (in biology) A dense structure present in many cells. Typically a single rounded structure encased within a membrane, the nucleus contains the genetic information.

organ     (in biology) Various parts of an organism that perform one or more particular functions. For instance, an ovary is an organ that makes eggs, the brain is an organ that makes sense of nerve signals and a plant’s roots are organs that take in nutrients and moisture.

organism     Any living thing, from elephants and plants to bacteria and other types of single-celled life.

scanning electron microscope     (also known as SEM) A scientific instrument in which the surface of a specimen is scanned by a beam of electrons that are reflected to form an image.

sea     An ocean (or region that is part of an ocean). Unlike lakes and streams, seawater — or ocean water — is salty.

slug     A soft-bodied invertebrate that travels by sliding across a surface of slime that its body releases. It resembles a snail without a shell.

species     A group of similar organisms capable of producing offspring that can survive and reproduce.

sperm     The reproductive cell produced by a male animal (or, in plants, produced by male organs). When one joins with an egg, the sperm cell initiates fertilization. This is the first step in creating a new organism.

spider     A type of arthropod with four pairs of legs that usually spin threads of silk that they can use to create webs or other structures.

testes     (singular: testis) Organs in the males of many animal species that makes sperm, the reproductive cells that fertilize eggs. This organ also is the primary site that makes testosterone, the primary male sex hormone.

unique     Something that is unlike anything else; the only one of its kind.

vacuum     Space with little or no matter in it. Laboratories or manufacturing plants may use vacuum equipment to pump out air, creating an area known as a vacuum chamber.

zoology     The study of animals and their habitats. Scientists who undertake this work are known as zoologists.

Citation

Journal: R.C. Neves et al. Microanatomy and development of the dwarf male of Symbion pandora (Phylum Cycliophora): New insights from ultrastructural investigation based on serial section electron microscopy. PLOS ONE. Vol. 10, April 15, 2015, p. e0122364. doi: 10.1371/journal.pone.0122364.

Journal: R.C. Neves et al. External morphology of the cycliophoran dwarf male: a comparative study of Symbion pandora and S. americanus. Helgoland Marine Research. Vol. 64, May 15, 2010, p. 257. doi: 10.1007/s10152-010-0204-5.

Journal: R.C. Neves et al. Cycliophoran dwarf males break the rule: high complexity with low cell numbers. The Biological Bulletin. Vol. 217, August 2009, p. 2. doi: 10.1086/BBLv217n1p2.

Journal: P. Funch et al. Symbiotic relations and feeding biology of Symbion pandora (Cycliophora) and Triticella flava (Bryozoa). Vie et Milieu — Life and Environment. Vol. 58, 2008, p. 185.

Journal: M. Obst et al. The microhabitat of Symbion pandora (Cycliophora) on the mouthparts of its host Nephrops norvegicus (Decapoda: Nephropidae). Marine Biology. Vol. 148, March 2006, p. 945. doi: 10.1007/s00227-005-0131-1.

Journal: M. Obst et al. Dwarf male of Symbion pandora (Cycliophora). Journal of Morphology. Vol. 255, January 6, 2003, p. 261. doi: 10.1002/jmor.10040.

Journal: P. Funch et al. Cycliophora is a new phylum with affinities to Entoprocta and Ectoprocta. Nature. Vol. 278, December 14, 1995, p. 711. doi: 10.1038/378711a0.