Two teens pull DNA from birds out of the air | Science News for Students

Two teens pull DNA from birds out of the air

Their new technique could help scientists track birds, using the DNA they leave behind
May 16, 2019 — 3:37 pm EST
a photo of a white and brown owl sitting in a tree

This is a brown hawk-owl, one of the species whose DNA two Japanese teens were able to detect with their new device. They unveiled that device at the 2019 Intel ISEF science competition.

Aaron Maizlish/Flickr (CC BY-NC 2.0)

PHOENIX, Ariz. — When animals travel, they leave bits of themselves behind. Fingerprints and feathers, skin cells and fur. Those bits contain DNA. For some time, scientists have been able to track down this environmental DNA — or eDNA — in soil and in water. Now, two teens have figured out how to also extract it from the air. They hope to use it to track rare birds — even ones people may never see.

Yuma Okamoto,17, and So Tsukamoto, 17, are seniors. Both attend Shizuoka Prefectural Kakegawa-Nishi High School in Kanegawa, Japan. They had previously detected eDNA in water. But when they heard their science teacher, a bird breeder, talk about the debris his animals left behind, the two began to think that eDNA might be in air, too. Their teacher had explained “that bird cages became very dirty with bird sebum,” Yuma says. Sebum (SEE-bum) is an oily substance that birds secrete and use to keep their feathers in good flying shape.

Birds also shed some sebum as they fly. Yuma and So reasoned that they might be able to detect where birds have been by scouting for the eDNA that had come from the sebum shed as a bird flew or perched.

In particular, they wanted to look for signs of owls. “They are nocturnal [active at night], and they have low population density,” Yuma notes. “It is difficult to spot them by eye.” So spotting owl eDNA in the air might be a very efficient way to find them. The teens designed a device that attaches to a tree trunk. It pulls in air and bubbles it through a liquid that collects DNA. Then, Yuma and So could take the solution back to their school and extract any eDNA it had picked up.

This is a Ural owl, a species that Yuma and So were able to detect with their device. 
Hans Hillewaert/Wikimedia Commons (CC-SA-3.0)

The teens tested their design first on white-cheeked starlings. These birds gather in large flocks, which suggested they should shed lots of eDNA. Yuma and So placed their detector in a spot where starlings liked to hang out. As a control — a part of the experiment where they expected no result — they placed their detector on a different day in some spot where starlings were rare. And the teens were able to show their device easily picked up starling eDNA when starlings were hanging around.

They also showed that the eDNA doesn’t last long. It breaks down quickly when exposed to sunlight and air. It also doesn’t travel far. Even when starlings were only 50 meters (164 feet) away from their perching site, their device could not detect them. 

So how well did the teens’ system work at detecting owls, which are much rarer in the environment? On 20 times, at 6 different locations, Yuma and So scouted for owl DNA. Each time, they would leave the detector out for 16 hours — plenty of time for an owl or two to fly by. As a control, the students got owl feathers from a local zoo and a museum. By testing those feathers, they knew what DNA they should be looking for. The teens were able to detect two different species — the Ural owl and the brown hawk-owl.

So and Yuma brought their stealthy bird detector to the Intel International Science and Engineering Fair. This yearly fair was created in 1950 by Society for Science & the Public, which still runs the event. (The Society also publishes Science News for Students.) This year, the Intel-sponsored fair brought together more than 1,800 students from 80 countries to share their science projects.

“In the future, this method could be applied to a lot of birds,” Yuma says of his new system. “We want to use this method for endangered species,” or species that are shy and hard to spot. The teens also hope to improve their device so scientists can catch birds as they fly by — even if they never see them at all.

a photo of Yuma Okamoto and So Tsukamoto holding a laptop showing their invention
Yuma Okamoto (left) and So Tsukamoto (right) show off a video of their invention that sucks air and bubbles through liquid to extract DNA that birds have left behind.
C. Ayers Photography/SSP

Power Words

(more about Power Words)

cell     The smallest structural and functional unit of an organism. Typically too small to see with the unaided eye, it consists of a watery fluid surrounded by a membrane or wall. Depending on their size, animals are made of anywhere from thousands to trillions of cells. Most organisms, such as yeasts, molds, bacteria and some algae, are composed of only one cell.

density     The measure of how condensed some object is, found by dividing its mass by its volume.

DNA     (short for deoxyribonucleic acid) A long, double-stranded and spiral-shaped molecule inside most living cells that carries genetic instructions. It is built on a backbone of phosphorus, oxygen, and carbon atoms. In all living things, from plants and animals to microbes, these instructions tell cells which molecules to make.

endangered     An adjective used to describe species at risk of going extinct.

engineering     The field of research that uses math and science to solve practical problems.

environment     The sum of all of the things that exist around some organism or the process and the condition those things create. Environment may refer to the weather and ecosystem in which some animal lives, or, perhaps, the temperature and humidity (or even the placement of things in the vicinity of an item of interest).

environmental DNA     (also known as eDNA )  DNA left in the environment by a species in the wild. Scientists can sometimes infer the presence of a species, and number of individuals in that part of the environment, solely from the genetic material (DNA) that they had left behind.

extract     (v.) To separate one chemical (or component of something) from a complex mix. (noun) A substance, often in concentrated form, that has been removed from its natural source. Extracts are often taken from plants (such as spearmint or lavender), flowers and buds (such as roses and cloves), fruit (such as lemons and oranges) or seeds and nuts (such as almonds and pistachios). Such extracts, sometimes used in cooking, often have very strong scents or flavors.

genetic     Having to do with chromosomes, DNA and the genes contained within DNA. The field of science dealing with these biological instructions is known as genetics. People who work in this field are geneticists.

high school     A designation for grades nine through 12 in the U.S. system of compulsory public education. High-school graduates may apply to colleges for further, advanced education.

Intel International Science and Engineering Fair (Intel ISEF)     Initially launched in 1950, this competition is one of three created (and still run) by the Society for Science & the Public. Each year now, approximately 1,800 high school students from more than 80 countries, regions, and territories are awarded the opportunity to showcase their independent research at Intel ISEF and compete for an average of almost $5 million in prizes. 

nocturnal     An adjective for something that is done, occurring or active at night.

population     (in biology) A group of individuals from the same species that lives in the same area.

preen     To make oneself look more attractive, such as by combing one’s hair or straightening the wrinkles out of one’s clothes. (in biology) A verb for the way birds straighten and clean their feathers.

sebum     A type of oil secreted by glands (sebaceous glands) in the skin. It helps keep the skin moist and healthy.

species     A group of similar organisms capable of producing offspring that can survive and reproduce.